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UTP-Na₂ CAS：285978-18-9
Our Uridine 5′-Triphosphate Disodium Salt (UTP disodium, UTP-Na₂) is premium grade pyrimidine nucleotide raw material exclusively manufactured for biotech labs, mRNA synthesis factories, pharmaceutical R&D and diagnostic reagent producers. With neutral pH 7.2–7.6, zero nuclease contamination and HPLC purity ≥98%, this disodium salt outperforms trisodium UTP for pH-sensitive in vitro transcription and cell culture assays. We support lab small trial orders and metric ton bulk wholesale with full QC documentation, stable cold chain global delivery.
UTP Disodium Salt Core Specifications
Exclusive Formulation
Pure CAS 285978-18-9 disodium salt, strictly distinct from trisodium UTP.
Pure CAS 285978-18-9 disodium salt, strictly distinct from trisodium UTP.
Optimized Neutral pH
Stable aqueous pH 7.2–7.6. No manual pH adjustment required for IVT.
Stable aqueous pH 7.2–7.6. No manual pH adjustment required for IVT.
Nuclease-Free Certified
RNase, DNase, protease & phosphatase: Not Detected.
RNase, DNase, protease & phosphatase: Not Detected.
Tiered HPLC Purity
≥98% (Molecular Biology Grade) / ≥99% (Pharmaceutical Grade).
≥98% (Molecular Biology Grade) / ≥99% (Pharmaceutical Grade).
Premium Physical State
White crystalline powder. Fully water-soluble with low hygroscopicity.
White crystalline powder. Fully water-soluble with low hygroscopicity.
Complete QC Audits
Full technical documentation set standard: COA, SDS, HPLC, & Heavy Metals.
Full technical documentation set standard: COA, SDS, HPLC, & Heavy Metals.
Flexible Packaging
Scalable options from 100mg–1kg lab bags up to 25kg industrial drums.
Scalable options from 100mg–1kg lab bags up to 25kg industrial drums.
Factory Direct Supply
Direct factory sourcing with custom sterile 100mM solution options.
Direct factory sourcing with custom sterile 100mM solution options.
Product Specs
Full Technical Specifications of Uridine 5′-Triphosphate Disodium Salt
Premium quality Uridine 5′-Triphosphate Disodium Salt (UTP Na₂) manufactured under strict quality control standards. Ideal for molecular biology, pharmaceutical manufacturing, and industrial biocatalysis applications.
Basic Chemical Identification & Properties
| Item | Standard Parameter |
|---|---|
| Full Chemical Name | Uridine 5′-Triphosphate Disodium Salt |
| Short Name | UTP Disodium / UTP Na2 |
| CAS Number | 285978-18-9 |
| Molecular Formula | C9H13N2Na2O15P3 |
| Exact Molecular Weight | 518.08 (anhydrous) |
| UV Absorption | λmax = 262 nm, ε = 10.0×10³ M⁻¹·cm⁻¹ (pH 7.0 Tris-HCl) |
| SMILES Code | O=P(O[Na])(OP(O)(OP(O)(O)=O)OC[C@H]1OC@@HC@H[C@@H]1O)O[Na] |
| Appearance | White to off-white crystalline lyophilized powder |
| Solubility | Freely soluble in deionized water, PBS, Tris buffer; insoluble in ethanol, acetone, ether |
| 100mM Aqueous pH Value | 7.2–7.6 |
| Decomposition Temp. | Above 140°C, gradual dephosphorylation into UDP / UMP |
| Hygroscopicity | Medium (far less moisture absorption than UTP trisodium salt) |
Grade-Based Purity & Impurity Control Standards
1. Molecular Biology Grade Lab & mRNA IVT Main Grade
- HPLC Assay: ≥98.0%
- UDP (Uridine Diphosphate): ≤1.0%
- UMP (Uridine Monophosphate): ≤0.5%
- Free Inorganic Phosphate: ≤0.3%
- Heavy Metals (Pb, As, Cd, Hg): ≤10 ppm
- Contaminants: RNase, DNase, Protease, Alkaline Phosphatase (Not Detected)
2. Pharmaceutical Intermediate Grade Ophthalmic Drug Raw Material
- HPLC Assay: ≥99.0%
- Total Related Nucleotides: ≤0.8%
- Loss on Drying: ≤5.0%
- Residue on Ignition: 18–22%
- Endotoxin: ≤0.1 EU/mg
3. Industrial Biocatalysis Grade Large-Scale Enzymatic Synthesis
- HPLC Assay: ≥95.0%
- UDP Impurity: ≤3.0% (No nuclease removal requirement)
Main Industrial & Laboratory Application Scenarios
- In Vitro Transcription (IVT): Core substrate for manufacturing mRNA, sgRNA, siRNA, and RNA probes (bulk buyer primary application).
- Cell Signaling Research: Functions as a P2Y2 / P2Y4 purinergic receptor agonist for epithelial mucosal repair and signal transduction studies.
- Pharmaceutical Manufacturing: Raw intermediate for ophthalmic nucleotide drugs targeting dry eye treatments.
- Enzymatic Biosynthesis: Premium substrate for glycosyltransferase enzyme activity testing and sugar nucleotide biosynthesis.
- Molecular Diagnostics: Essential raw material for fluorescent quantitative PCR (qPCR) and RNA diagnostic kits.
- CRISPR Gene Editing: Critical supporting substrate for in vitro RNA synthesis in CRISPR-based gene editing workflows.
Packaging & Global Logistics Information
- Small Trial Packaging: 100mg, 250mg, 500mg, 1g vacuum aluminum foil bags with desiccant.
- Medium Bulk Lab Packaging: 5kg sealed foil bags.
- Industrial Mass Production Packaging: 25kg fiber drum with double PE inner liner + aluminum foil inner bag.
- Custom Liquid Service: 100mM sterile UTP disodium aqueous solution, available in 1mL–1000mL sterile vials.
- Shipping Condition: Dry ice cold transport for both powder & liquid formulations in moisture-proof sealed packages.
- Minimum Order Quantity (MOQ): 100mg for laboratory testing; 1kg for factory pilot production; 25kg for mass commercial manufacturing.
Storage, Shelf Life & Handling Guidance
Storage & Stability:
- Long-term Storage (Unopened Powder): Store at -20°C, light-proof airtight seal. Shelf life: 36 months.
- Short-term Storage (Within 30 Days): Store at 2–8°C in a refrigerated dry environment. Validity: 12 months.
- 100mM Liquid Solution: Store at -20°C aliquoted. Crucial: Avoid repeated freeze-thaw cycles. Validity: 12 months.
Professional Handling Tips:
Always wear nitrile gloves & safety goggles during operation. Avoid dust inhalation. Keep away from acidic volatile gases to prevent chemical degradation.
Factory QC Support Documents (B2B Trust Signal)
We supply a full set of factory test documents for every single shipment without extra fees:
Certificate of Analysis (COA)
Material Safety Data Sheet (MSDS)
HPLC Chromatogram
Heavy Metal Inspection Report
Endotoxin Test Record
Nuclease Residual Test Report
Material Safety Data Sheet (MSDS)
HPLC Chromatogram
Heavy Metal Inspection Report
Endotoxin Test Record
Nuclease Residual Test Report
FAQ
Certificate
COA, MSDS, and GMP PDF files are available for download.
Ensure quality compliance for pharmaceutical, biotechnology, and cosmetic applications.
Uridine 5′-Triphosphate Disodium Salt Complete Guide for mRNA IVT & Biotech Bulk Buyers
Executive Summary: Comprehensive procurement and technical manual of UTP Disodium Salt (CAS 285978-18-9) for global biotech procurement managers, mRNA manufacturing facilities, and pharmaceutical R&D teams.
Introduction to Uridine 5′-Triphosphate Disodium Salt (UTP Na₂ CAS 285978-18-9)
Uridine 5′-Triphosphate Disodium Salt, commonly shortened as UTP disodium or UTP Na₂, is a vital pyrimidine nucleoside triphosphate raw material widely demanded by global biotech laboratories, mRNA manufacturing plants, pharmaceutical R&D teams, and diagnostic reagent developers. Distinguished from trisodium UTP by its unique CAS Registry Number 285978-18-9, this disodium nucleotide salt delivers a neutral pH aqueous environment that eliminates reaction interference in pH-sensitive biological experiments—a core advantage attracting bulk procurement managers from North America, Europe, Southeast Asia, and Australia.
As a fundamental building block for all RNA strands synthesized via in vitro transcription (IVT), UTP disodium pairs with ATP, GTP, and CTP to assemble complete mRNA, sgRNA, siRNA, and RNA probe molecules for gene therapy, vaccine research, and CRISPR genome editing workflows. Unlike free acid UTP which lacks water solubility and chemical stability, the disodium salt crystalline powder features excellent water solubility and controlled hygroscopicity, simplifying storage, weighing, and liquid preparation for high-volume industrial production lines.
⚠️ Global Procurement Challenges Addressed:
Global biotech purchasers often face challenges selecting qualified nucleotide suppliers: inconsistent purity, hidden nuclease contamination, unstable pH values, and insufficient cold chain shipping solutions. This comprehensive guide breaks down UTP disodium core advantages, grade classifications, real-world industrial applications, storage protocols, and factory bulk purchasing tips to help procurement teams make data-driven sourcing decisions.
Global biotech purchasers often face challenges selecting qualified nucleotide suppliers: inconsistent purity, hidden nuclease contamination, unstable pH values, and insufficient cold chain shipping solutions. This comprehensive guide breaks down UTP disodium core advantages, grade classifications, real-world industrial applications, storage protocols, and factory bulk purchasing tips to help procurement teams make data-driven sourcing decisions.
Key Chemical & Physical Advantages of UTP Disodium Salt vs. Trisodium UTP
The most frequent confusion among nucleotide buyers lies in differentiating UTP disodium and trisodium salt variants, a detail that directly impacts downstream experiment success and finished product quality. UTP disodium salt carries two sodium cations in its molecular structure, producing a neutral 7.2–7.6 pH value when dissolved in deionized water. This natural neutral state matches standard IVT reaction buffer conditions, removing the need for manual pH adjustment that risks introducing foreign ions and lowering RNA synthesis yield.
UTP trisodium salt, by contrast, contains three sodium ions and generates an alkaline solution ranging from pH 8.0 to 8.8. For mammalian cell culture and therapeutic mRNA manufacturing, excess alkalinity disrupts RNA polymerase activity and increases mRNA immunogenicity, leading to failed transcription batches and wasted raw material costs. Another critical benefit of UTP disodium is reduced moisture absorption: trisodium UTP clumps rapidly under normal room humidity, while disodium powder maintains a free-flow crystalline texture for accurate weighing over long-term storage.
Additional Physical Strengths of Factory-Grade UTP Disodium:
- Clear UV Absorption: Peak absorption at 262 nm for quick and precise concentration quantification.
- Optimized Solubility: Complete solubility in aqueous biological buffers; zero solubility in organic solvents like ethanol, enabling simple post-reaction purification steps without nucleotide loss.
- Consistent Molecular Weight: Anhydrous molecular weight of 518.08 delivers consistent molar concentration calculations for standardized lab protocols, eliminating concentration deviation caused by variable water content.
Three Main Product Grades of UTP Disodium for Different B2B Application Scenarios
Our factory manufactures three tiered UTP disodium grades tailored to distinct buyer production demands, covering small academic lab research, pharmaceutical intermediate synthesis, and large-scale industrial biocatalysis. Each grade holds strict impurity thresholds verified via HPLC, UV spectrophotometry, and biological contamination testing.
1. Molecular Biology Grade UTP Disodium
Primary for mRNA IVT & Cell Research
This is the highest-demand grade among university labs, biotech startups, and mRNA pilot production facilities. With HPLC purity ≥98% and non-detectable RNase, DNase, protease, and phosphatase residuals, it supports sensitive RNA synthesis, P2Y purinergic receptor cell signaling assays, and CRISPR sgRNA production.
Heavy metal limits < 10 ppm | Batch COA Included | MOQ from 100mg
2. Pharmaceutical Intermediate Grade UTP Disodium
Ophthalmic Drug Raw Material
Formulated for commercial nucleotide eye drug manufacturing, this grade raises HPLC purity to ≥99% with total related nucleotide impurities capped at 0.8%. Endotoxin levels are controlled below 0.1 EU/mg to avoid human ocular tissue irritation in finished dry eye treatment formulations.
Ideal for Diquafosol Sodium synthesis | Full Regulatory QC Files for FDA/EMA
3. Industrial Biocatalysis Grade UTP Disodium
Large-Scale Enzymatic Synthesis
Designed for bulk sugar nucleotide biosynthesis and high-throughput enzyme activity screening, this grade maintains ≥95% HPLC purity with relaxed nuclease control standards to reduce raw material costs for mass production factories. Available in bulk 25kg fiber drums.
Bulk 25kg Fiber Drum Packaging | Highly Cost-Effective for Large-Scale Lines
Top Industrial & Laboratory Use Cases for Bulk UTP Disodium Procurement
B2B buyers sourcing UTP disodium fall into four core industry segments, each with unique quality and packaging requirements we fully accommodate as a direct nucleotide factory supplier:
- mRNA IVT Manufacturing: Represents the largest consumption market. mRNA vaccine developers, gene therapy companies, and custom RNA synthesis service labs purchase kilogram-scale UTP disodium to prepare complete NTP mixes for T7, T3, and SP6 RNA polymerase transcription reactions. Neutral pH disodium salt stabilizes RNA strand formation, boosting transcription yield by 10–15% compared to trisodium alternatives.
- Cell Biology & Purinergic Receptor Research: Laboratories utilize UTP disodium as a selective P2Y₂ and P2Y₄ receptor agonist. Researchers studying airway epithelial repair, neurogenesis, and inflammation pathways rely on nuclease-free UTP disodium to trigger controlled cellular signaling responses without background contamination skewing experimental data.
- Ophthalmic Pharmaceutical Manufacturing: Companies integrate pharmaceutical-grade UTP disodium as the key starting material for dry eye therapeutic drugs. UTP binds to ocular surface P2Y receptors to stimulate mucin secretion. Our low-endotoxin variant complies with global regulations enforced by authorities like the U.S. Food and Drug Administration (FDA) and the European Medicines Agency (EMA).
- Diagnostic Reagents & Enzyme Engineering: Factories purchase industrial-grade UTP disodium for qPCR kits, RNA detection test strips, and glycosyltransferase substrate screening. Custom sterile 100mM liquid UTP disodium eliminates in-house powder dissolution steps, streamlining high-throughput reagent assembly lines.
Critical Storage & Shipping Protocols to Preserve Product Stability
Improper storage and transportation are the leading causes of nucleotide degradation. The triphosphate structure breaks down under high temperature, moisture, and repeated freeze-thaw cycles, splitting into UDP and UMP impurities that render batches unusable for sensitive RNA synthesis.
Strict Storage Temperature Rules:
- Unopened Powder (Vacuum Sealed): Long-term storage at -20°C in light-proof dry cabinets preserves full activity for up to 36 months. Short-term refrigerated storage (2–8°C) is valid for up to 12 months.
- Opened Powder Handling: Must be aliquoted into small centrifuge tubes inside a low-humidity glove box, sealed tightly, and returned to -20°C. Exposed powder degrades within one week at room temperature.
- Pre-made 100mM Liquid Solution: Requires aliquoting into single-use vials before freezing. Repeated freeze-thaw cycles accelerate phosphate chain cleavage. Valid for 12 months at -20°C (maximum 3 thaw cycles recommended).
Global shipping follows strict cold chain standards: Small lab samples ship via international express (DHL/FedEx) with dry ice insulation, while 25kg industrial drum bulk orders utilize air freight with temperature-controlled cargo containers. All packages feature double moisture barrier layers to block humid air during transit.
B2B Bulk Purchasing Tips for UTP Disodium From Direct Factory Suppliers
Sourcing UTP disodium directly from nucleotide manufacturers delivers significant cost and quality advantages over third-party reagent distributors. Sourcing managers should evaluate four key considerations:
1. CAS Verification
Always confirm the exact CAS number 285978-18-9 to avoid receiving trisodium UTP (CAS 19817-92-6) by mistake, a common supplier mix-up. Always request a pre-shipment HPLC chromatogram.
Always confirm the exact CAS number 285978-18-9 to avoid receiving trisodium UTP (CAS 19817-92-6) by mistake, a common supplier mix-up. Always request a pre-shipment HPLC chromatogram.
2. Grade Matching
Match product grade to your core application to optimize costs. Academic labs can select molecular biology grade, while large mRNA factories benefit from volume discounts on 5kg/25kg bulk pharmaceutical orders.
Match product grade to your core application to optimize costs. Academic labs can select molecular biology grade, while large mRNA factories benefit from volume discounts on 5kg/25kg bulk pharmaceutical orders.
3. Custom Formulation
Inquire about custom formulation support, including pre-made sterile 100mM UTP solutions, tailored purity specifications, and private-label OEM packaging options. Free testing samples are available for qualified buyers.
Inquire about custom formulation support, including pre-made sterile 100mM UTP solutions, tailored purity specifications, and private-label OEM packaging options. Free testing samples are available for qualified buyers.
4. Document Full Set
Validate documentation availability. Reliable suppliers must provide COA, MSDS, HPLC spectra, heavy metal, and nuclease test records without additional fees, crucial for customs clearance and audits.
Validate documentation availability. Reliable suppliers must provide COA, MSDS, HPLC spectra, heavy metal, and nuclease test records without additional fees, crucial for customs clearance and audits.
Conclusion
Uridine 5′-Triphosphate Disodium Salt (CAS 285978-18-9) remains the gold-standard nucleotide raw material for modern mRNA manufacturing, cell biology research, and ophthalmic drug synthesis due to its neutral pH, low hygroscopicity, and ultra-low biological contamination profiles. Selecting the correct disodium grade, implementing standardized cold storage protocols, and partnering with a certified direct factory supplier eliminates common sourcing pain points. Whether you require milligram-scale lab trial samples or metric-ton industrial bulk supply, our tiered UTP disodium product range and full technical QC support meet all global biotech and pharmaceutical buyer quality standards, ensuring robust and reliable RNA production workflows worldwide.
